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pancreatic organoid growth medium stem cell #06040  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc pancreatic organoid growth medium stem cell #06040
Lineage tracing of SETD4 + cells in P3 and 8 weeks SETD4-CreER T2 ; Rosa26 mTmG/ + mice. ( a ) Schedule and lineage tracing of SETD4 + cells in P3 SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Neonatal mice were treated with 2 consecutive doses of TAM and then analyzed at 2 time points: 24 h or 8 weeks post-induction with TAM. Representative immunofluorescence and quantification of single (1), doublet (2), and clones of multi-cells (≥ 3) at the respective time points are shown. Solid arrows: single cell (1), hollow arrows: multi-cell (≥ 3) clones. Scale bar in the lineage, 80 μm. ( b ) Quantification for Ki67 and PCNA of GFP + recombinant cells in the acinar, duct or islet compartments from adult pancreases. ( c ) Schedule of lineage tracing of SETD4 + cells in 8 weeks SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Adult mice were treated with 2 consecutive doses of TAM and then analyzed at 4 time points: 24 h, 1, 4 and 10 months post-induction with TAM. ( d ) Representative immunofluorescence for amylase, CK19, insulin, glucagon, somatostatin and ghrelin in pancreas of adult (8 weeks) mice pancreases at 4 months after TAM-induction. Scale bar for all merged images, 15 μm. Scale bar for all spilt images, 10 μm. ( e ) Quantification of recombinant GFP + cells of total (DAPI + <t>)</t> <t>pancreatic</t> cells in adult (P56) mice 1 month and 4 months after TAM-induction. ( f ) Representative immunofluorescence of lineage tracing of SETD4 + cells 10 months after TAM-induction in adult SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Solid arrows: single cell (1), hollow arrows: multi-cell (≥ 3) clones. Scale bar in the lineage, 40 μm. ( g ) Isolation by fluorescence activated cell sorting (FACS) of GFP + and GFP - cells from SETD4-CreER T2 ; Rosa26 mTmG/ + mice at 24 h after TAM-induction. GFP + and GFP - cells in duct compartment were sorted and then cultured in 3-Dimentional <t>Matrigel.</t> Representative formation of cell-organized organoids by SETD4 + cells (green) in a 3-Dimentional Matrigel-based culture over a month. Scale bar for each image, 15 μm. All data are represented as mean ± SD. * p < 0.05. *** p < 0.001. ns not significant. n = 4 mice. Arrows indicate recombinant GFP + cells (green). Nuclei were stained with DAPI.
Pancreatic Organoid Growth Medium Stem Cell #06040, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/full+organoid+growth+medium/pancreatic+organoid+growth+medium+stem+cell++06040/pmc08206148-260-4-9
Average 90 stars, based on 1 article reviews
pancreatic organoid growth medium stem cell #06040 - by Bioz Stars, 2026-10
90/100 stars
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insticult organoid growth medium  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc insticult organoid growth medium
Lineage tracing of SETD4 + cells in P3 and 8 weeks SETD4-CreER T2 ; Rosa26 mTmG/ + mice. ( a ) Schedule and lineage tracing of SETD4 + cells in P3 SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Neonatal mice were treated with 2 consecutive doses of TAM and then analyzed at 2 time points: 24 h or 8 weeks post-induction with TAM. Representative immunofluorescence and quantification of single (1), doublet (2), and clones of multi-cells (≥ 3) at the respective time points are shown. Solid arrows: single cell (1), hollow arrows: multi-cell (≥ 3) clones. Scale bar in the lineage, 80 μm. ( b ) Quantification for Ki67 and PCNA of GFP + recombinant cells in the acinar, duct or islet compartments from adult pancreases. ( c ) Schedule of lineage tracing of SETD4 + cells in 8 weeks SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Adult mice were treated with 2 consecutive doses of TAM and then analyzed at 4 time points: 24 h, 1, 4 and 10 months post-induction with TAM. ( d ) Representative immunofluorescence for amylase, CK19, insulin, glucagon, somatostatin and ghrelin in pancreas of adult (8 weeks) mice pancreases at 4 months after TAM-induction. Scale bar for all merged images, 15 μm. Scale bar for all spilt images, 10 μm. ( e ) Quantification of recombinant GFP + cells of total (DAPI + <t>)</t> <t>pancreatic</t> cells in adult (P56) mice 1 month and 4 months after TAM-induction. ( f ) Representative immunofluorescence of lineage tracing of SETD4 + cells 10 months after TAM-induction in adult SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Solid arrows: single cell (1), hollow arrows: multi-cell (≥ 3) clones. Scale bar in the lineage, 40 μm. ( g ) Isolation by fluorescence activated cell sorting (FACS) of GFP + and GFP - cells from SETD4-CreER T2 ; Rosa26 mTmG/ + mice at 24 h after TAM-induction. GFP + and GFP - cells in duct compartment were sorted and then cultured in 3-Dimentional <t>Matrigel.</t> Representative formation of cell-organized organoids by SETD4 + cells (green) in a 3-Dimentional Matrigel-based culture over a month. Scale bar for each image, 15 μm. All data are represented as mean ± SD. * p < 0.05. *** p < 0.001. ns not significant. n = 4 mice. Arrows indicate recombinant GFP + cells (green). Nuclei were stained with DAPI.
Insticult Organoid Growth Medium, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/full+organoid+growth+medium/insticult+organoid+growth+medium/pm39432783-378-5-9
Average 90 stars, based on 1 article reviews
insticult organoid growth medium - by Bioz Stars, 2026-10
90/100 stars
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intesticult organoid growth medium basal medium  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc intesticult organoid growth medium basal medium
Lineage tracing of SETD4 + cells in P3 and 8 weeks SETD4-CreER T2 ; Rosa26 mTmG/ + mice. ( a ) Schedule and lineage tracing of SETD4 + cells in P3 SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Neonatal mice were treated with 2 consecutive doses of TAM and then analyzed at 2 time points: 24 h or 8 weeks post-induction with TAM. Representative immunofluorescence and quantification of single (1), doublet (2), and clones of multi-cells (≥ 3) at the respective time points are shown. Solid arrows: single cell (1), hollow arrows: multi-cell (≥ 3) clones. Scale bar in the lineage, 80 μm. ( b ) Quantification for Ki67 and PCNA of GFP + recombinant cells in the acinar, duct or islet compartments from adult pancreases. ( c ) Schedule of lineage tracing of SETD4 + cells in 8 weeks SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Adult mice were treated with 2 consecutive doses of TAM and then analyzed at 4 time points: 24 h, 1, 4 and 10 months post-induction with TAM. ( d ) Representative immunofluorescence for amylase, CK19, insulin, glucagon, somatostatin and ghrelin in pancreas of adult (8 weeks) mice pancreases at 4 months after TAM-induction. Scale bar for all merged images, 15 μm. Scale bar for all spilt images, 10 μm. ( e ) Quantification of recombinant GFP + cells of total (DAPI + <t>)</t> <t>pancreatic</t> cells in adult (P56) mice 1 month and 4 months after TAM-induction. ( f ) Representative immunofluorescence of lineage tracing of SETD4 + cells 10 months after TAM-induction in adult SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Solid arrows: single cell (1), hollow arrows: multi-cell (≥ 3) clones. Scale bar in the lineage, 40 μm. ( g ) Isolation by fluorescence activated cell sorting (FACS) of GFP + and GFP - cells from SETD4-CreER T2 ; Rosa26 mTmG/ + mice at 24 h after TAM-induction. GFP + and GFP - cells in duct compartment were sorted and then cultured in 3-Dimentional <t>Matrigel.</t> Representative formation of cell-organized organoids by SETD4 + cells (green) in a 3-Dimentional Matrigel-based culture over a month. Scale bar for each image, 15 μm. All data are represented as mean ± SD. * p < 0.05. *** p < 0.001. ns not significant. n = 4 mice. Arrows indicate recombinant GFP + cells (green). Nuclei were stained with DAPI.
Intesticult Organoid Growth Medium Basal Medium, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/full+organoid+growth+medium/intesticult+organoid+growth+medium/pm39729509-175-14-21
Average 90 stars, based on 1 article reviews
intesticult organoid growth medium basal medium - by Bioz Stars, 2026-10
90/100 stars
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murine intesticult organoid growth medium  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc murine intesticult organoid growth medium
Lineage tracing of SETD4 + cells in P3 and 8 weeks SETD4-CreER T2 ; Rosa26 mTmG/ + mice. ( a ) Schedule and lineage tracing of SETD4 + cells in P3 SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Neonatal mice were treated with 2 consecutive doses of TAM and then analyzed at 2 time points: 24 h or 8 weeks post-induction with TAM. Representative immunofluorescence and quantification of single (1), doublet (2), and clones of multi-cells (≥ 3) at the respective time points are shown. Solid arrows: single cell (1), hollow arrows: multi-cell (≥ 3) clones. Scale bar in the lineage, 80 μm. ( b ) Quantification for Ki67 and PCNA of GFP + recombinant cells in the acinar, duct or islet compartments from adult pancreases. ( c ) Schedule of lineage tracing of SETD4 + cells in 8 weeks SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Adult mice were treated with 2 consecutive doses of TAM and then analyzed at 4 time points: 24 h, 1, 4 and 10 months post-induction with TAM. ( d ) Representative immunofluorescence for amylase, CK19, insulin, glucagon, somatostatin and ghrelin in pancreas of adult (8 weeks) mice pancreases at 4 months after TAM-induction. Scale bar for all merged images, 15 μm. Scale bar for all spilt images, 10 μm. ( e ) Quantification of recombinant GFP + cells of total (DAPI + <t>)</t> <t>pancreatic</t> cells in adult (P56) mice 1 month and 4 months after TAM-induction. ( f ) Representative immunofluorescence of lineage tracing of SETD4 + cells 10 months after TAM-induction in adult SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Solid arrows: single cell (1), hollow arrows: multi-cell (≥ 3) clones. Scale bar in the lineage, 40 μm. ( g ) Isolation by fluorescence activated cell sorting (FACS) of GFP + and GFP - cells from SETD4-CreER T2 ; Rosa26 mTmG/ + mice at 24 h after TAM-induction. GFP + and GFP - cells in duct compartment were sorted and then cultured in 3-Dimentional <t>Matrigel.</t> Representative formation of cell-organized organoids by SETD4 + cells (green) in a 3-Dimentional Matrigel-based culture over a month. Scale bar for each image, 15 μm. All data are represented as mean ± SD. * p < 0.05. *** p < 0.001. ns not significant. n = 4 mice. Arrows indicate recombinant GFP + cells (green). Nuclei were stained with DAPI.
Murine Intesticult Organoid Growth Medium, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/full+organoid+growth+medium/intesticult+mouse+organoid+growth+medium/pmc10835974-89-14-19
Average 90 stars, based on 1 article reviews
murine intesticult organoid growth medium - by Bioz Stars, 2026-10
90/100 stars
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human intesticult organoid media  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc human intesticult organoid media
Lineage tracing of SETD4 + cells in P3 and 8 weeks SETD4-CreER T2 ; Rosa26 mTmG/ + mice. ( a ) Schedule and lineage tracing of SETD4 + cells in P3 SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Neonatal mice were treated with 2 consecutive doses of TAM and then analyzed at 2 time points: 24 h or 8 weeks post-induction with TAM. Representative immunofluorescence and quantification of single (1), doublet (2), and clones of multi-cells (≥ 3) at the respective time points are shown. Solid arrows: single cell (1), hollow arrows: multi-cell (≥ 3) clones. Scale bar in the lineage, 80 μm. ( b ) Quantification for Ki67 and PCNA of GFP + recombinant cells in the acinar, duct or islet compartments from adult pancreases. ( c ) Schedule of lineage tracing of SETD4 + cells in 8 weeks SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Adult mice were treated with 2 consecutive doses of TAM and then analyzed at 4 time points: 24 h, 1, 4 and 10 months post-induction with TAM. ( d ) Representative immunofluorescence for amylase, CK19, insulin, glucagon, somatostatin and ghrelin in pancreas of adult (8 weeks) mice pancreases at 4 months after TAM-induction. Scale bar for all merged images, 15 μm. Scale bar for all spilt images, 10 μm. ( e ) Quantification of recombinant GFP + cells of total (DAPI + <t>)</t> <t>pancreatic</t> cells in adult (P56) mice 1 month and 4 months after TAM-induction. ( f ) Representative immunofluorescence of lineage tracing of SETD4 + cells 10 months after TAM-induction in adult SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Solid arrows: single cell (1), hollow arrows: multi-cell (≥ 3) clones. Scale bar in the lineage, 40 μm. ( g ) Isolation by fluorescence activated cell sorting (FACS) of GFP + and GFP - cells from SETD4-CreER T2 ; Rosa26 mTmG/ + mice at 24 h after TAM-induction. GFP + and GFP - cells in duct compartment were sorted and then cultured in 3-Dimentional <t>Matrigel.</t> Representative formation of cell-organized organoids by SETD4 + cells (green) in a 3-Dimentional Matrigel-based culture over a month. Scale bar for each image, 15 μm. All data are represented as mean ± SD. * p < 0.05. *** p < 0.001. ns not significant. n = 4 mice. Arrows indicate recombinant GFP + cells (green). Nuclei were stained with DAPI.
Human Intesticult Organoid Media, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/full+organoid+growth+medium/human+intesticulttm+organoid+growth+medium/pm40059828-200-19-24
Average 90 stars, based on 1 article reviews
human intesticult organoid media - by Bioz Stars, 2026-10
90/100 stars
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pancreacult organoid growth medium stemcell #06040  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc pancreacult organoid growth medium stemcell #06040
Lineage tracing of SETD4 + cells in P3 and 8 weeks SETD4-CreER T2 ; Rosa26 mTmG/ + mice. ( a ) Schedule and lineage tracing of SETD4 + cells in P3 SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Neonatal mice were treated with 2 consecutive doses of TAM and then analyzed at 2 time points: 24 h or 8 weeks post-induction with TAM. Representative immunofluorescence and quantification of single (1), doublet (2), and clones of multi-cells (≥ 3) at the respective time points are shown. Solid arrows: single cell (1), hollow arrows: multi-cell (≥ 3) clones. Scale bar in the lineage, 80 μm. ( b ) Quantification for Ki67 and PCNA of GFP + recombinant cells in the acinar, duct or islet compartments from adult pancreases. ( c ) Schedule of lineage tracing of SETD4 + cells in 8 weeks SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Adult mice were treated with 2 consecutive doses of TAM and then analyzed at 4 time points: 24 h, 1, 4 and 10 months post-induction with TAM. ( d ) Representative immunofluorescence for amylase, CK19, insulin, glucagon, somatostatin and ghrelin in pancreas of adult (8 weeks) mice pancreases at 4 months after TAM-induction. Scale bar for all merged images, 15 μm. Scale bar for all spilt images, 10 μm. ( e ) Quantification of recombinant GFP + cells of total (DAPI + <t>)</t> <t>pancreatic</t> cells in adult (P56) mice 1 month and 4 months after TAM-induction. ( f ) Representative immunofluorescence of lineage tracing of SETD4 + cells 10 months after TAM-induction in adult SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Solid arrows: single cell (1), hollow arrows: multi-cell (≥ 3) clones. Scale bar in the lineage, 40 μm. ( g ) Isolation by fluorescence activated cell sorting (FACS) of GFP + and GFP - cells from SETD4-CreER T2 ; Rosa26 mTmG/ + mice at 24 h after TAM-induction. GFP + and GFP - cells in duct compartment were sorted and then cultured in 3-Dimentional <t>Matrigel.</t> Representative formation of cell-organized organoids by SETD4 + cells (green) in a 3-Dimentional Matrigel-based culture over a month. Scale bar for each image, 15 μm. All data are represented as mean ± SD. * p < 0.05. *** p < 0.001. ns not significant. n = 4 mice. Arrows indicate recombinant GFP + cells (green). Nuclei were stained with DAPI.
Pancreacult Organoid Growth Medium Stemcell #06040, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/full+organoid+growth+medium/pancreaculttm+organoid+growth+medium/pm35505283-42-9-13
Average 90 stars, based on 1 article reviews
pancreacult organoid growth medium stemcell #06040 - by Bioz Stars, 2026-10
90/100 stars
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intesticult organoid growth medium (human) ogmh  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc intesticult organoid growth medium (human) ogmh
Lineage tracing of SETD4 + cells in P3 and 8 weeks SETD4-CreER T2 ; Rosa26 mTmG/ + mice. ( a ) Schedule and lineage tracing of SETD4 + cells in P3 SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Neonatal mice were treated with 2 consecutive doses of TAM and then analyzed at 2 time points: 24 h or 8 weeks post-induction with TAM. Representative immunofluorescence and quantification of single (1), doublet (2), and clones of multi-cells (≥ 3) at the respective time points are shown. Solid arrows: single cell (1), hollow arrows: multi-cell (≥ 3) clones. Scale bar in the lineage, 80 μm. ( b ) Quantification for Ki67 and PCNA of GFP + recombinant cells in the acinar, duct or islet compartments from adult pancreases. ( c ) Schedule of lineage tracing of SETD4 + cells in 8 weeks SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Adult mice were treated with 2 consecutive doses of TAM and then analyzed at 4 time points: 24 h, 1, 4 and 10 months post-induction with TAM. ( d ) Representative immunofluorescence for amylase, CK19, insulin, glucagon, somatostatin and ghrelin in pancreas of adult (8 weeks) mice pancreases at 4 months after TAM-induction. Scale bar for all merged images, 15 μm. Scale bar for all spilt images, 10 μm. ( e ) Quantification of recombinant GFP + cells of total (DAPI + <t>)</t> <t>pancreatic</t> cells in adult (P56) mice 1 month and 4 months after TAM-induction. ( f ) Representative immunofluorescence of lineage tracing of SETD4 + cells 10 months after TAM-induction in adult SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Solid arrows: single cell (1), hollow arrows: multi-cell (≥ 3) clones. Scale bar in the lineage, 40 μm. ( g ) Isolation by fluorescence activated cell sorting (FACS) of GFP + and GFP - cells from SETD4-CreER T2 ; Rosa26 mTmG/ + mice at 24 h after TAM-induction. GFP + and GFP - cells in duct compartment were sorted and then cultured in 3-Dimentional <t>Matrigel.</t> Representative formation of cell-organized organoids by SETD4 + cells (green) in a 3-Dimentional Matrigel-based culture over a month. Scale bar for each image, 15 μm. All data are represented as mean ± SD. * p < 0.05. *** p < 0.001. ns not significant. n = 4 mice. Arrows indicate recombinant GFP + cells (green). Nuclei were stained with DAPI.
Intesticult Organoid Growth Medium (Human) Ogmh, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/full+organoid+growth+medium/intesticult+organoid+growth+medium+ogm/pmc10891043-165-19-26
Average 90 stars, based on 1 article reviews
intesticult organoid growth medium (human) ogmh - by Bioz Stars, 2026-10
90/100 stars
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pancreaculttm organoid growth mouse medium  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc pancreaculttm organoid growth mouse medium
Lineage tracing of SETD4 + cells in P3 and 8 weeks SETD4-CreER T2 ; Rosa26 mTmG/ + mice. ( a ) Schedule and lineage tracing of SETD4 + cells in P3 SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Neonatal mice were treated with 2 consecutive doses of TAM and then analyzed at 2 time points: 24 h or 8 weeks post-induction with TAM. Representative immunofluorescence and quantification of single (1), doublet (2), and clones of multi-cells (≥ 3) at the respective time points are shown. Solid arrows: single cell (1), hollow arrows: multi-cell (≥ 3) clones. Scale bar in the lineage, 80 μm. ( b ) Quantification for Ki67 and PCNA of GFP + recombinant cells in the acinar, duct or islet compartments from adult pancreases. ( c ) Schedule of lineage tracing of SETD4 + cells in 8 weeks SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Adult mice were treated with 2 consecutive doses of TAM and then analyzed at 4 time points: 24 h, 1, 4 and 10 months post-induction with TAM. ( d ) Representative immunofluorescence for amylase, CK19, insulin, glucagon, somatostatin and ghrelin in pancreas of adult (8 weeks) mice pancreases at 4 months after TAM-induction. Scale bar for all merged images, 15 μm. Scale bar for all spilt images, 10 μm. ( e ) Quantification of recombinant GFP + cells of total (DAPI + <t>)</t> <t>pancreatic</t> cells in adult (P56) mice 1 month and 4 months after TAM-induction. ( f ) Representative immunofluorescence of lineage tracing of SETD4 + cells 10 months after TAM-induction in adult SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Solid arrows: single cell (1), hollow arrows: multi-cell (≥ 3) clones. Scale bar in the lineage, 40 μm. ( g ) Isolation by fluorescence activated cell sorting (FACS) of GFP + and GFP - cells from SETD4-CreER T2 ; Rosa26 mTmG/ + mice at 24 h after TAM-induction. GFP + and GFP - cells in duct compartment were sorted and then cultured in 3-Dimentional <t>Matrigel.</t> Representative formation of cell-organized organoids by SETD4 + cells (green) in a 3-Dimentional Matrigel-based culture over a month. Scale bar for each image, 15 μm. All data are represented as mean ± SD. * p < 0.05. *** p < 0.001. ns not significant. n = 4 mice. Arrows indicate recombinant GFP + cells (green). Nuclei were stained with DAPI.
Pancreaculttm Organoid Growth Mouse Medium, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/full+organoid+growth+medium/pancreaculttm+organoid+growth+mouse+medium/pm37996891-63-5-10
Average 90 stars, based on 1 article reviews
pancreaculttm organoid growth mouse medium - by Bioz Stars, 2026-10
90/100 stars
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organoid growth medium  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc organoid growth medium
Lineage tracing of SETD4 + cells in P3 and 8 weeks SETD4-CreER T2 ; Rosa26 mTmG/ + mice. ( a ) Schedule and lineage tracing of SETD4 + cells in P3 SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Neonatal mice were treated with 2 consecutive doses of TAM and then analyzed at 2 time points: 24 h or 8 weeks post-induction with TAM. Representative immunofluorescence and quantification of single (1), doublet (2), and clones of multi-cells (≥ 3) at the respective time points are shown. Solid arrows: single cell (1), hollow arrows: multi-cell (≥ 3) clones. Scale bar in the lineage, 80 μm. ( b ) Quantification for Ki67 and PCNA of GFP + recombinant cells in the acinar, duct or islet compartments from adult pancreases. ( c ) Schedule of lineage tracing of SETD4 + cells in 8 weeks SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Adult mice were treated with 2 consecutive doses of TAM and then analyzed at 4 time points: 24 h, 1, 4 and 10 months post-induction with TAM. ( d ) Representative immunofluorescence for amylase, CK19, insulin, glucagon, somatostatin and ghrelin in pancreas of adult (8 weeks) mice pancreases at 4 months after TAM-induction. Scale bar for all merged images, 15 μm. Scale bar for all spilt images, 10 μm. ( e ) Quantification of recombinant GFP + cells of total (DAPI + <t>)</t> <t>pancreatic</t> cells in adult (P56) mice 1 month and 4 months after TAM-induction. ( f ) Representative immunofluorescence of lineage tracing of SETD4 + cells 10 months after TAM-induction in adult SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Solid arrows: single cell (1), hollow arrows: multi-cell (≥ 3) clones. Scale bar in the lineage, 40 μm. ( g ) Isolation by fluorescence activated cell sorting (FACS) of GFP + and GFP - cells from SETD4-CreER T2 ; Rosa26 mTmG/ + mice at 24 h after TAM-induction. GFP + and GFP - cells in duct compartment were sorted and then cultured in 3-Dimentional <t>Matrigel.</t> Representative formation of cell-organized organoids by SETD4 + cells (green) in a 3-Dimentional Matrigel-based culture over a month. Scale bar for each image, 15 μm. All data are represented as mean ± SD. * p < 0.05. *** p < 0.001. ns not significant. n = 4 mice. Arrows indicate recombinant GFP + cells (green). Nuclei were stained with DAPI.
Organoid Growth Medium, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/full+organoid+growth+medium/organoid+growth+medium/pmc11302876-266-12-15
Average 90 stars, based on 1 article reviews
organoid growth medium - by Bioz Stars, 2026-10
90/100 stars
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mouse hepaticulttm organoid growth medium  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc mouse hepaticulttm organoid growth medium
Lineage tracing of SETD4 + cells in P3 and 8 weeks SETD4-CreER T2 ; Rosa26 mTmG/ + mice. ( a ) Schedule and lineage tracing of SETD4 + cells in P3 SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Neonatal mice were treated with 2 consecutive doses of TAM and then analyzed at 2 time points: 24 h or 8 weeks post-induction with TAM. Representative immunofluorescence and quantification of single (1), doublet (2), and clones of multi-cells (≥ 3) at the respective time points are shown. Solid arrows: single cell (1), hollow arrows: multi-cell (≥ 3) clones. Scale bar in the lineage, 80 μm. ( b ) Quantification for Ki67 and PCNA of GFP + recombinant cells in the acinar, duct or islet compartments from adult pancreases. ( c ) Schedule of lineage tracing of SETD4 + cells in 8 weeks SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Adult mice were treated with 2 consecutive doses of TAM and then analyzed at 4 time points: 24 h, 1, 4 and 10 months post-induction with TAM. ( d ) Representative immunofluorescence for amylase, CK19, insulin, glucagon, somatostatin and ghrelin in pancreas of adult (8 weeks) mice pancreases at 4 months after TAM-induction. Scale bar for all merged images, 15 μm. Scale bar for all spilt images, 10 μm. ( e ) Quantification of recombinant GFP + cells of total (DAPI + <t>)</t> <t>pancreatic</t> cells in adult (P56) mice 1 month and 4 months after TAM-induction. ( f ) Representative immunofluorescence of lineage tracing of SETD4 + cells 10 months after TAM-induction in adult SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Solid arrows: single cell (1), hollow arrows: multi-cell (≥ 3) clones. Scale bar in the lineage, 40 μm. ( g ) Isolation by fluorescence activated cell sorting (FACS) of GFP + and GFP - cells from SETD4-CreER T2 ; Rosa26 mTmG/ + mice at 24 h after TAM-induction. GFP + and GFP - cells in duct compartment were sorted and then cultured in 3-Dimentional <t>Matrigel.</t> Representative formation of cell-organized organoids by SETD4 + cells (green) in a 3-Dimentional Matrigel-based culture over a month. Scale bar for each image, 15 μm. All data are represented as mean ± SD. * p < 0.05. *** p < 0.001. ns not significant. n = 4 mice. Arrows indicate recombinant GFP + cells (green). Nuclei were stained with DAPI.
Mouse Hepaticulttm Organoid Growth Medium, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/full+organoid+growth+medium/mouse+hepaticulttm+organoid+growth+medium/pmc10040241-147-11-16
Average 90 stars, based on 1 article reviews
mouse hepaticulttm organoid growth medium - by Bioz Stars, 2026-10
90/100 stars
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organoid growth medium human ogm-h  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc organoid growth medium human ogm-h
Lineage tracing of SETD4 + cells in P3 and 8 weeks SETD4-CreER T2 ; Rosa26 mTmG/ + mice. ( a ) Schedule and lineage tracing of SETD4 + cells in P3 SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Neonatal mice were treated with 2 consecutive doses of TAM and then analyzed at 2 time points: 24 h or 8 weeks post-induction with TAM. Representative immunofluorescence and quantification of single (1), doublet (2), and clones of multi-cells (≥ 3) at the respective time points are shown. Solid arrows: single cell (1), hollow arrows: multi-cell (≥ 3) clones. Scale bar in the lineage, 80 μm. ( b ) Quantification for Ki67 and PCNA of GFP + recombinant cells in the acinar, duct or islet compartments from adult pancreases. ( c ) Schedule of lineage tracing of SETD4 + cells in 8 weeks SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Adult mice were treated with 2 consecutive doses of TAM and then analyzed at 4 time points: 24 h, 1, 4 and 10 months post-induction with TAM. ( d ) Representative immunofluorescence for amylase, CK19, insulin, glucagon, somatostatin and ghrelin in pancreas of adult (8 weeks) mice pancreases at 4 months after TAM-induction. Scale bar for all merged images, 15 μm. Scale bar for all spilt images, 10 μm. ( e ) Quantification of recombinant GFP + cells of total (DAPI + <t>)</t> <t>pancreatic</t> cells in adult (P56) mice 1 month and 4 months after TAM-induction. ( f ) Representative immunofluorescence of lineage tracing of SETD4 + cells 10 months after TAM-induction in adult SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Solid arrows: single cell (1), hollow arrows: multi-cell (≥ 3) clones. Scale bar in the lineage, 40 μm. ( g ) Isolation by fluorescence activated cell sorting (FACS) of GFP + and GFP - cells from SETD4-CreER T2 ; Rosa26 mTmG/ + mice at 24 h after TAM-induction. GFP + and GFP - cells in duct compartment were sorted and then cultured in 3-Dimentional <t>Matrigel.</t> Representative formation of cell-organized organoids by SETD4 + cells (green) in a 3-Dimentional Matrigel-based culture over a month. Scale bar for each image, 15 μm. All data are represented as mean ± SD. * p < 0.05. *** p < 0.001. ns not significant. n = 4 mice. Arrows indicate recombinant GFP + cells (green). Nuclei were stained with DAPI.
Organoid Growth Medium Human Ogm H, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
organoid growth medium human ogm-h - by Bioz Stars, 2026-10
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hepaticult medium  (STEMCELL Technologies Inc)
90
STEMCELL Technologies Inc hepaticult medium
Lineage tracing of SETD4 + cells in P3 and 8 weeks SETD4-CreER T2 ; Rosa26 mTmG/ + mice. ( a ) Schedule and lineage tracing of SETD4 + cells in P3 SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Neonatal mice were treated with 2 consecutive doses of TAM and then analyzed at 2 time points: 24 h or 8 weeks post-induction with TAM. Representative immunofluorescence and quantification of single (1), doublet (2), and clones of multi-cells (≥ 3) at the respective time points are shown. Solid arrows: single cell (1), hollow arrows: multi-cell (≥ 3) clones. Scale bar in the lineage, 80 μm. ( b ) Quantification for Ki67 and PCNA of GFP + recombinant cells in the acinar, duct or islet compartments from adult pancreases. ( c ) Schedule of lineage tracing of SETD4 + cells in 8 weeks SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Adult mice were treated with 2 consecutive doses of TAM and then analyzed at 4 time points: 24 h, 1, 4 and 10 months post-induction with TAM. ( d ) Representative immunofluorescence for amylase, CK19, insulin, glucagon, somatostatin and ghrelin in pancreas of adult (8 weeks) mice pancreases at 4 months after TAM-induction. Scale bar for all merged images, 15 μm. Scale bar for all spilt images, 10 μm. ( e ) Quantification of recombinant GFP + cells of total (DAPI + <t>)</t> <t>pancreatic</t> cells in adult (P56) mice 1 month and 4 months after TAM-induction. ( f ) Representative immunofluorescence of lineage tracing of SETD4 + cells 10 months after TAM-induction in adult SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Solid arrows: single cell (1), hollow arrows: multi-cell (≥ 3) clones. Scale bar in the lineage, 40 μm. ( g ) Isolation by fluorescence activated cell sorting (FACS) of GFP + and GFP - cells from SETD4-CreER T2 ; Rosa26 mTmG/ + mice at 24 h after TAM-induction. GFP + and GFP - cells in duct compartment were sorted and then cultured in 3-Dimentional <t>Matrigel.</t> Representative formation of cell-organized organoids by SETD4 + cells (green) in a 3-Dimentional Matrigel-based culture over a month. Scale bar for each image, 15 μm. All data are represented as mean ± SD. * p < 0.05. *** p < 0.001. ns not significant. n = 4 mice. Arrows indicate recombinant GFP + cells (green). Nuclei were stained with DAPI.
Hepaticult Medium, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/full+organoid+growth+medium/hepaticult+organoid+growth+medium/pm40540118-35-2-11
Average 90 stars, based on 1 article reviews
hepaticult medium - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

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Lineage tracing of SETD4 + cells in P3 and 8 weeks SETD4-CreER T2 ; Rosa26 mTmG/ + mice. ( a ) Schedule and lineage tracing of SETD4 + cells in P3 SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Neonatal mice were treated with 2 consecutive doses of TAM and then analyzed at 2 time points: 24 h or 8 weeks post-induction with TAM. Representative immunofluorescence and quantification of single (1), doublet (2), and clones of multi-cells (≥ 3) at the respective time points are shown. Solid arrows: single cell (1), hollow arrows: multi-cell (≥ 3) clones. Scale bar in the lineage, 80 μm. ( b ) Quantification for Ki67 and PCNA of GFP + recombinant cells in the acinar, duct or islet compartments from adult pancreases. ( c ) Schedule of lineage tracing of SETD4 + cells in 8 weeks SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Adult mice were treated with 2 consecutive doses of TAM and then analyzed at 4 time points: 24 h, 1, 4 and 10 months post-induction with TAM. ( d ) Representative immunofluorescence for amylase, CK19, insulin, glucagon, somatostatin and ghrelin in pancreas of adult (8 weeks) mice pancreases at 4 months after TAM-induction. Scale bar for all merged images, 15 μm. Scale bar for all spilt images, 10 μm. ( e ) Quantification of recombinant GFP + cells of total (DAPI + ) pancreatic cells in adult (P56) mice 1 month and 4 months after TAM-induction. ( f ) Representative immunofluorescence of lineage tracing of SETD4 + cells 10 months after TAM-induction in adult SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Solid arrows: single cell (1), hollow arrows: multi-cell (≥ 3) clones. Scale bar in the lineage, 40 μm. ( g ) Isolation by fluorescence activated cell sorting (FACS) of GFP + and GFP - cells from SETD4-CreER T2 ; Rosa26 mTmG/ + mice at 24 h after TAM-induction. GFP + and GFP - cells in duct compartment were sorted and then cultured in 3-Dimentional Matrigel. Representative formation of cell-organized organoids by SETD4 + cells (green) in a 3-Dimentional Matrigel-based culture over a month. Scale bar for each image, 15 μm. All data are represented as mean ± SD. * p < 0.05. *** p < 0.001. ns not significant. n = 4 mice. Arrows indicate recombinant GFP + cells (green). Nuclei were stained with DAPI.

Journal: Scientific Reports

Article Title: SETD4-expressing cells contribute to pancreatic development and response to cerulein induced pancreatitis injury

doi: 10.1038/s41598-021-92075-5

Figure Lengend Snippet: Lineage tracing of SETD4 + cells in P3 and 8 weeks SETD4-CreER T2 ; Rosa26 mTmG/ + mice. ( a ) Schedule and lineage tracing of SETD4 + cells in P3 SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Neonatal mice were treated with 2 consecutive doses of TAM and then analyzed at 2 time points: 24 h or 8 weeks post-induction with TAM. Representative immunofluorescence and quantification of single (1), doublet (2), and clones of multi-cells (≥ 3) at the respective time points are shown. Solid arrows: single cell (1), hollow arrows: multi-cell (≥ 3) clones. Scale bar in the lineage, 80 μm. ( b ) Quantification for Ki67 and PCNA of GFP + recombinant cells in the acinar, duct or islet compartments from adult pancreases. ( c ) Schedule of lineage tracing of SETD4 + cells in 8 weeks SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Adult mice were treated with 2 consecutive doses of TAM and then analyzed at 4 time points: 24 h, 1, 4 and 10 months post-induction with TAM. ( d ) Representative immunofluorescence for amylase, CK19, insulin, glucagon, somatostatin and ghrelin in pancreas of adult (8 weeks) mice pancreases at 4 months after TAM-induction. Scale bar for all merged images, 15 μm. Scale bar for all spilt images, 10 μm. ( e ) Quantification of recombinant GFP + cells of total (DAPI + ) pancreatic cells in adult (P56) mice 1 month and 4 months after TAM-induction. ( f ) Representative immunofluorescence of lineage tracing of SETD4 + cells 10 months after TAM-induction in adult SETD4-CreER T2 ; Rosa26 mTmG/ + mice. Solid arrows: single cell (1), hollow arrows: multi-cell (≥ 3) clones. Scale bar in the lineage, 40 μm. ( g ) Isolation by fluorescence activated cell sorting (FACS) of GFP + and GFP - cells from SETD4-CreER T2 ; Rosa26 mTmG/ + mice at 24 h after TAM-induction. GFP + and GFP - cells in duct compartment were sorted and then cultured in 3-Dimentional Matrigel. Representative formation of cell-organized organoids by SETD4 + cells (green) in a 3-Dimentional Matrigel-based culture over a month. Scale bar for each image, 15 μm. All data are represented as mean ± SD. * p < 0.05. *** p < 0.001. ns not significant. n = 4 mice. Arrows indicate recombinant GFP + cells (green). Nuclei were stained with DAPI.

Article Snippet: After gelation of the Matrigel, pancreatic organoid growth medium (Stem Cell Technologies #06040) was added in each well and replenished every 3 days.

Techniques: Immunofluorescence, Clone Assay, Recombinant, Isolation, Fluorescence, FACS, Cell Culture, Staining